Anticorps Polyclonal de lapin anti-APOB

APOB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(53)

N° de cat : 20578-1-AP

Synonymes

Apolipoprotein B, Apo B, Apo B-100, ApoB100, ApoB48

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Galerie de données de validation

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  WB analysis using 20578-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20578-1-AP

  mouse liver and human plasma were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20578-1-AP

  HEK-293 cells were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20578-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 20578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using APOB antibody (20578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 20578-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using APOB antibody (20578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: plasma humain, cellules HEK-293, tissu hépatique de souris, tissu plasmatique humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 46 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

20578-1-AP cible APOB dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: apolipoprotein B (including Ag(x) antigen)
• Masse moléculaire calculée: 516 kDa
• Poids moléculaire observé: 150-250 kDa, 400-520 kDa
• Numéro d’acquisition GenBank: NM_000384
• Symbole du gène: APOB
• Identification du gène (NCBI): 338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

Publications

Species	Application	Title
mouse,human	WB	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
mouse	WB	Nat Metab; Liver RBFOX2 regulates cholesterol homeostasis via Scarb1 alternative splicing in mice; Authors - Helen A B Paterson
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu

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