Anticorps Polyclonal de lapin anti-APOB

APOB Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20578-1-PBS

Synonymes

Apolipoprotein B, Apo B, Apo B-100, ApoB100, ApoB48

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Galerie de données de validation

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  WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

  mouse liver and human plasma were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  WB analysis using 20578-1-AP (same clone as 20578-1-PBS)

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 20578-1-AP (same clone as 20578-1-PBS)

  HEK-293 cells were subjected to Tris-acetate gel system followed by western blot with 20578-1-AP (APOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 20578-1-AP (same clone as 20578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 20578-1-AP (same clone as 20578-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20578-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 20578-1-AP (same clone as 20578-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using APOB antibody (20578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 20578-1-AP (same clone as 20578-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using APOB antibody (20578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20578-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20578-1-PBS cible APOB dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: apolipoprotein B (including Ag(x) antigen)
• Masse moléculaire calculée: 516 kDa
• Poids moléculaire observé: 150-250 kDa, 400-520 kDa
• Numéro d’acquisition GenBank: NM_000384
• Symbole du gène: APOB
• Identification du gène (NCBI): 338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

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