Anticorps Polyclonal de lapin anti-APOB

APOB Polyclonal Antibody for WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB

Conjugaison

HRP

Avis (1)

N° de cat : HRP-20578

Synonymes

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB

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Galerie de données de validation

• 

  WB analysis of human plasma using HRP-20578

  human plasma were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using HRP-20578

  mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using HRP-20578

  mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

HRP-20578 cible APOB dans les applications de WB et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: apolipoprotein B (including Ag(x) antigen)
• Masse moléculaire calculée: 516 kDa
• Poids moléculaire observé: 150-250 kDa, 400-520 kDa
• Numéro d’acquisition GenBank: NM_000384
• Symbole du gène: APOB
• Identification du gène (NCBI): 338
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tony (Verified Customer) (08-28-2025)

The Western Blot shows a band corresponding to APOB, detected using the HRP-conjugated polyclonal antibody. The signal intensity confirms the effective recognition of the target protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

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