Anticorps Monoclonal anti-APOD

APOD Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C6D10

N° de cat : 66215-1-PBS

Synonymes

1C6D10, Apo D, Apo-D, apolipoprotein D

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Galerie de données de validation

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  WB analysis of human plasma using 66215-1-Ig (same clone as 66215-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  WB analysis using 66215-1-Ig (same clone as 66215-1-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66215-1-Ig (same clone as 66215-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66215-1-Ig (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66215-1-Ig (same clone as 66215-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using APOD antibody (66215-1-Ig, Clone: 1C6D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66215-1-Ig (same clone as 66215-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOD (66215-1-Ig, Clone:1C6D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66215-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66215-1-PBS cible APOD dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOD Protéine recombinante Ag21422
• Nom complet: apolipoprotein D
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC007402
• Symbole du gène: APOD
• Identification du gène (NCBI): 347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

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