Anticorps Monoclonal anti-APOE

• Phare
• Validé par KD/KO

APOE Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B2C9

N° de cat : 66830-1-PBS

Synonymes

1B2C9, Apo E, Apo-E, apoe4, apolipoprotein E

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Galerie de données de validation

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  WB analysis of human plasma using 66830-1-Ig (same clone as 66830-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  WB result of APOE antibody (66830-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66830-1-Ig (same clone as 66830-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66830-1-Ig (same clone as 66830-1-PBS)

  human blood was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66830-1-Ig (same clone as 66830-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug APOE Monoclonal antibody (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 66830-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66830-1-PBS cible APOE dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOE Protéine recombinante Ag28186
• Nom complet: Apolipoprotein E
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC003557
• Symbole du gène: APOE
• Identification du gène (NCBI): 348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

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