Anticorps Recombinant de lapin anti-APOE

APOE Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242310B3

N° de cat : 84775-6-PBS

Synonymes

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

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Galerie de données de validation

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  WB analysis using 84775-6-RR (same clone as 84775-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84775-6-RR (APOE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84775-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84775-6-RR (same clone as 84775-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOE Recombinant antibody (84775-6-RR, Clone:242310B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84775-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84775-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84775-6-RR against Human APOE were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84775-6-PBS cible APOE dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: Apolipoprotein E
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-38 kDa
• Numéro d’acquisition GenBank: BC003557
• Symbole du gène: APOE
• Identification du gène (NCBI): 348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).

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