Anticorps Recombinant de lapin anti-APOH

APOH Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242454E4

N° de cat : 84943-2-PBS

Synonymes

Apolipoprotein H, 242454E4, Activated protein C-binding protein, Apo H, Apo-H

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Galerie de données de validation

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  WB analysis of human plasma using 84943-2-RR (same clone as 84943-2-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84943-2-RR (APOH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01667-1

  Sandwich ELISA standard curve of MP01667-1, Human APOH Recombinant Matched Antibody Pair - PBS only. 84943-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3084. 84943-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP01667-1

  Serum of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 69.62 µg/mL with a range of 55.50 - 88.74 µg/mL.

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  Sample test of MP01667-1

  Plasma of six individual healthy human donors was measured. The human APOH concentration of detected samples was determined to be 51.16 µg/mL with a range of 38.03 - 72.18 µg/mL.

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  Sample test of MP01667-1

  The mean APOH concentration was determined to be 21.74 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

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  IHC staining of human liver cancer using 84943-2-RR (same clone as 84943-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84943-2-RR (APOH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 84943-2-RR (same clone as 84943-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84943-2-RR (APOH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84943-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01667-1

  Cytometric bead array standard curve of MP01667-1, APOH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84943-1-PBS. Detection antibody: 84943-2-PBS. Standard: Eg3084. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84943-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84943-2-RR against Human APOH were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84943-2-PBS cible APOH dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APOH Protéine recombinante Eg3084
• Nom complet: apolipoprotein H (beta-2-glycoprotein I)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 43-55 kDa
• Numéro d’acquisition GenBank: NM_000042.2
• Symbole du gène: APOH
• Identification du gène (NCBI): 350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.

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