Anticorps Recombinant de lapin anti-APOL1

APOL1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240814D11

N° de cat : 83770-5-RR

Synonymes

Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11

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Galerie de données de validation

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  WB analysis using 83770-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83770-5-RR (APOL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83770-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1 antibody (83770-5-RR, Clone: 240814D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83770-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83770-5-RR against Human APOL1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A375, cellules HepG2, cellules MCF-7, plasma humain
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83770-5-RR cible APOL1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APOL1 Protéine recombinante ag2016
• Nom complet: apolipoprotein L, 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC017331
• Symbole du gène: APOL1
• Identification du gène (NCBI): 8542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue.

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