Anticorps Monoclonal anti-APOL1-Specific

• Phare
• Validé par KD/KO

APOL1-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1G12D11

N° de cat : 66124-1-PBS

Synonymes

APOL1, 1G12D11, Apo-L, ApoL-I, Apolipoprotein L I

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Galerie de données de validation

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  WB analysis of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 66124-1-Ig (same clone as 66124-1-PBS)

  IP result of anti-APOL1-Specific (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66124-1-Ig (same clone as 66124-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1-Specific antibody (66124-1-Ig, Clone: 1G12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66124-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66124-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66124-1-Ig against Human APOL1 were performed. The Affinity Constant is below 1 pM.

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Informations sur le produit

66124-1-PBS cible APOL1-Specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOL1-Specific Protéine recombinante Ag2016
• Nom complet: apolipoprotein L, 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC017331
• Symbole du gène: APOL1
• Identification du gène (NCBI): 8542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

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