Anticorps Polyclonal de lapin anti-APOM

APOM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12817-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 12817-1-AP (same clone as 12817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 12817-1-AP (same clone as 12817-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 12817-1-AP (same clone as 12817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12817-1-AP (same clone as 12817-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12817-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12817-1-PBS cible APOM dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APOM Protéine recombinante Ag3137
• Nom complet: apolipoprotein M
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC020683
• Symbole du gène: APOM
• Identification du gène (NCBI): 55937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

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