Anticorps Monoclonal anti-APP

• Phare
• Validé par KD/KO

APP Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5C2A1

N° de cat : 60342-1-PBS

Synonymes

amyloid precursor protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42, AB40

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Galerie de données de validation

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  WB analysis using 60342-1-Ig (same clone as 60342-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 60342-1-Ig (same clone as 60342-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60342-1-Ig (same clone as 60342-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (Beta Amyloid antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60342-1-PBS cible APP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat

• Réactivité: Humain, Lapin, porc, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APP Protéine recombinante Ag0769
• Nom complet: amyloid beta (A4) precursor protein
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC004369
• Symbole du gène: APP
• Identification du gène (NCBI): 351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).

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