Anticorps Recombinant de lapin anti-APP

APP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250546B3

N° de cat : 84829-6-RR

Synonymes

amyloid precursor protein, A4 amyloid protein, AB40, AB42, AD1

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Galerie de données de validation

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  WB analysis using 84829-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84829-6-RR (APP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 84829-6-RR

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84829-6-RR (APP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 84829-6-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using APP antibody (84829-6-RR, Clone: 250546B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84829-6-RR cible APP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APP Protéine recombinante Eg3070
• Nom complet: amyloid beta (A4) precursor protein
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_000484.3
• Symbole du gène: APP
• Identification du gène (NCBI): 351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).

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