Anticorps Polyclonal de lapin anti-APRIL,TNFSF13

• Phare
• Validé par KD/KO

APRIL,TNFSF13 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10680-1-AP

Synonymes

TNFSF13, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TALL2, CD256

---

Galerie de données de validation

• 

  WB analysis of Raji using 10680-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10680-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10680-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10680-1-AP (TNFSF13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10680-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10680-1-AP (TNFSF13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules Raji
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

10680-1-AP cible APRIL,TNFSF13 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APRIL,TNFSF13 Protéine recombinante Ag1056
• Nom complet: tumor necrosis factor (ligand) superfamily, member 13
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC008042
• Symbole du gène: TNFSF13
• Identification du gène (NCBI): 8741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.

Publications

Species	Application	Title
human	IHC	Front Immunol; TNFSF13 Is a Novel Onco-Inflammatory Marker and Correlates With Immune Infiltration in Gliomas.; Authors - Rui Chen
human	WB	Clin Biochem; APRIL knockdown suppresses migration and invasion of human colon carcinoma cells.; Authors - Ding Weifeng W ; KD Validated
human	WB	Pathol Oncol Res; Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway.; Authors - Ding Weifeng W ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}