Anticorps Recombinant de lapin anti-APRIL/TNFSF13

APRIL/TNFSF13 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

241666F9

N° de cat : 98331-2-PBS

Synonymes

241666F9, A proliferation-inducing ligand, APRIL, CD256, Tnfsf13

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98331-2-RR (same clone as 98331-2-PBS)

  1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone: 241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98331-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98331-2-RR (same clone as 98331-2-PBS)

  1x10^6 HEK-293T cells or APRIL/TNFSF13 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse APRIL/TNFSF13 Rabbit RecAb (98331-2-RR, Clone:241666F9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98331-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98331-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98331-2-RR against Mouse APRIL/TNFSF13 were performed. The affinity constant is 0.228 nM.

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Informations sur le produit

98331-2-PBS cible APRIL/TNFSF13 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: APRIL/TNFSF13 Protéine recombinante Eg1561
• Nom complet: tumor necrosis factor (ligand) superfamily, member 13
• Masse moléculaire calculée: 27kDa
• Numéro d’acquisition GenBank: NM_023517.2
• Symbole du gène: Tnfsf13
• Identification du gène (NCBI): 69583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

TNFSF13 gene encodes tumor necrosis factor ligand superfamily member 13 (or APRIL) belonging to the tumor necrosis factor (TNF) family. APRIL is highly expressed in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages, and its precursor is cleaved by furin. APRIL may be implicated in the regulation of tumor cell growth and also monocyte/macrophage-mediated immunological processes.

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