Anticorps Monoclonal anti-AQP1

AQP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5D6

N° de cat : 66805-1-PBS

Synonymes

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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Galerie de données de validation

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  WB analysis of human heart using 66805-1-Ig (same clone as 66805-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66805-1-Ig (same clone as 66805-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66805-1-Ig (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66805-1-Ig (AQP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 66805-1-Ig (same clone as 66805-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AQP1 antibody (66805-1-Ig, Clone: 2E5D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66805-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66805-1-PBS cible AQP1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AQP1 Protéine recombinante Ag14093
• Nom complet: aquaporin 1 (Colton blood group)
• Masse moléculaire calculée: 269 aa, 29 kDa
• Poids moléculaire observé: 28 kDa, 38-40 kDa
• Numéro d’acquisition GenBank: BC022486
• Symbole du gène: AQP1
• Identification du gène (NCBI): 358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

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