Anticorps Recombinant de lapin anti-AQP2

AQP2 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242886B8

N° de cat : 85402-1-PBS

Synonymes

Aquaporin 2, AQP-2, Aquaporin2

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Galerie de données de validation

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  WB analysis using 85402-1-RR (same clone as 85402-1-PBS)

  Unboiled mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85402-1-RR (AQP2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85402-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85402-1-RR (same clone as 85402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85402-1-RR (AQP2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85402-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85402-1-RR (same clone as 85402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85402-1-RR (AQP2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85402-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01925-2

  Cytometric bead array standard curve of MP01925-2, AQP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85402-1-PBS. Detection antibody: 85402-3-PBS. Standard: Ag29931. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP01925-1

  Cytometric bead array standard curve of MP01925-1, AQP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85402-1-PBS. Detection antibody: 85402-2-PBS. Standard: Ag29931. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 85402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85402-1-RR against Human AQP2 were performed. The affinity constant is 0.955 nM.

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Informations sur le produit

85402-1-PBS cible AQP2 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AQP2 Protéine recombinante Ag29931
• Nom complet: aquaporin 2 (collecting duct)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 28-29 kDa, 35-38 kDa
• Numéro d’acquisition GenBank: NM_000486
• Symbole du gène: AQP2
• Identification du gène (NCBI): 359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.

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