Anticorps Monoclonal anti-Aquaporin 4

Aquaporin 4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G7D3

N° de cat : 68448-1-PBS

Synonymes

AQP4, Aquaporin-4, Aquaporin4, AQP-4, AQP 4

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Galerie de données de validation

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  WB analysis using 68448-1-Ig (same clone as 68448-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spinal cord using 68448-1-Ig (same clone as 68448-1-PBS)

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 68448-1-Ig (AQP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68448-1-Ig (same clone as 68448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68448-1-Ig (Aquaporin 4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68448-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68448-1-PBS cible Aquaporin 4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Aquaporin 4 Protéine recombinante Ag9830
• Nom complet: Aquaporin 4
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC022286
• Symbole du gène: Aquaporin 4
• Identification du gène (NCBI): 361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

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