Anticorps Recombinant de lapin anti-Aquaporin 4

Aquaporin 4 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240856C7

N° de cat : 82296-6-PBS

Synonymes

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

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Galerie de données de validation

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  WB analysis using 82296-6-RR (same clone as 82296-6-PBS)

  37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 82296-6-RR (same clone as 82296-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (82296-6-RR, Clone: 240856C7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82296-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

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Informations sur le produit

82296-6-PBS cible Aquaporin 4 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Aquaporin 4 Protéine recombinante Ag9561
• Nom complet: Aquaporin 4
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 32-34 kDa
• Numéro d’acquisition GenBank: BC022286
• Symbole du gène: Aquaporin 4
• Identification du gène (NCBI): 361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

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