Anticorps Polyclonal de lapin anti-AQP5

AQP5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 20334-1-AP

Synonymes

AQP 5, AQP5, aquaporin 5

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Galerie de données de validation

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  WB analysis using 20334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 20334-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

20334-1-AP cible AQP5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AQP5 Protéine recombinante Ag14103
• Nom complet: aquaporin 5
• Masse moléculaire calculée: 265 aa, 28 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC032946
• Symbole du gène: AQP5
• Identification du gène (NCBI): 362
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Ezrin Is a Novel Protein Partner of Aquaporin-5 in Human Salivary Glands and Shows Altered Expression and Cellular Localization in Sjögren's Syndrome; Authors - Clara Chivasso
human,mouse	WB,IHC	J Ethnopharmacol; Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway.; Authors - Xingbao Tao
	IF	Front Genet; A New Homotetramer Hemoglobin in the Pulmonary Surfactant of Plateau Zokors (Myospalax Baileyi).; Authors - Jimei Li
rat	IF,WB	Front Immunol; D-tagatose protects against oleic acid-induced acute respiratory distress syndrome in rats by activating PTEN/PI3K/AKT pathway; Authors - Jian Huang
rat	IF	Cell Rep; A homotetrameric hemoglobin expressed in alveolar epithelial cells increases blood oxygenation in high-altitude plateau pika (Ochotona curzoniae); Authors - Zhifang An
mouse	IF	Biomaterials; Inhaled pulmonary surfactant biomimetic liposomes for reversing idiopathic pulmonary fibrosis through synergistic therapeutic strategy; Authors - Binghua Wang