Anticorps Monoclonal anti-androgen receptor

androgen receptor Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1F7C12

N° de cat : 66747-1-Ig

Synonymes

AIS, androgen receptor, AR, DHTR, Dihydrotestosterone receptor, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM

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Galerie de données de validation

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  WB analysis using 66747-1-Ig

  PC-3(AR-), DU 145(AR-) and LNCaP (AR+) cell lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66747-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66747-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66747-1-Ig (AR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66747-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66747-1-Ig (AR antibody) at dilution of 1:8000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of LNCaP using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using androgen receptor antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human prostate cancer using 66747-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using AR antibody (66747-1-Ig, Clone: 1F7C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules NCCIT, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de la prostate humain, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:600-1:3000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

66747-1-Ig cible androgen receptor dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: androgen receptor Protéine recombinante Ag17291
• Nom complet: androgen receptor
• Masse moléculaire calculée: 914 aa, 99 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC132975
• Symbole du gène: AR
• Identification du gène (NCBI): 367
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )

Publications

Species	Application	Title
rat	IHC	Development; Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues; Authors - Parmveer Singh
rat	WB	Chin J Nat Med; Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro; Authors - Qi Huang
human,mouse	WB,IF,IP	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song
human	WB,IF	Gynecol Endocrinol; N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway; Authors - Yuan-Xue Jing