Anticorps Polyclonal de lapin anti-ARC/ARG3.1

• Phare
• Validé par KD/KO

ARC/ARG3.1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(28)

Avis (2)

N° de cat : 16290-1-AP

Synonymes

ARC, ARC/ARG3.1, Arg3.1, KIAA0278

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 16290-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16290-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16290-1-AP

  IP result of anti-ARC/ARG3.1 (IP:16290-1-AP, 4ug; Detection:16290-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

16290-1-AP cible ARC/ARG3.1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARC/ARG3.1 Protéine recombinante Ag9445
• Nom complet: activity-regulated cytoskeleton-associated protein
• Masse moléculaire calculée: 396 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC012321
• Symbole du gène: ARC
• Identification du gène (NCBI): 23237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration.

Publications

Species	Application	Title
human	WB	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
rat	WB,IHC	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
human	WB	Aging (Albany NY); Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.; Authors - Chenhaoyi Xu
human	WB	Neurobiol Dis; Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus.; Authors - Daniel Ojeda-Juarez
mouse	IF,WB,IP	Front Mol Neurosci; Activity-Dependent Arc Expression and Homeostatic Synaptic Plasticity Are Altered in Neurons from a Mouse Model of Angelman Syndrome.; Authors - Elissa D Pastuzyn

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Georgiana (Verified Customer) (01-04-2023)

For the image, magenta is Arc and cyan is GFAP

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain tissue, 20um slices

ARC/ARG3.1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, 20um slices (Cat no:16290-1-AP)

FH

Xin (Verified Customer) (01-23-2022)

Although there are some non specific bands, there is a major band at the expected 50 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

ARC/ARG3.1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:16290-1-AP)

{{ptg:RelatedPrimaryAntibodies}}