Anticorps Monoclonal anti-ARC

• Phare
• Validé par KD/KO

ARC Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G4B5

N° de cat : 66550-1-PBS

Synonymes

hArc, Arg3.1, ARC/ARG3.1, Activity-regulated gene 3.1 protein homolog, Activity-regulated cytoskeleton-associated protein

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Galerie de données de validation

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  WB analysis using 66550-1-Ig (same clone as 66550-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66550-1-Ig (ARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66550-1-Ig (same clone as 66550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66550-1-Ig (same clone as 66550-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 66550-1-Ig (same clone as 66550-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ARC antibody (66550-1-Ig, Clone: 1G4B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66550-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66550-1-PBS cible ARC dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARC Protéine recombinante Ag9445
• Nom complet: activity-regulated cytoskeleton-associated protein
• Masse moléculaire calculée: 396 aa, 45 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC012321
• Symbole du gène: ARC
• Identification du gène (NCBI): 23237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.

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