Anticorps Monoclonal anti-Amphiregulin

Amphiregulin Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1A1G9

N° de cat : 66433-1-Ig

Synonymes

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

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Galerie de données de validation

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  WB analysis of A549 using 66433-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66433-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66433-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66433-1-Ig cible Amphiregulin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Amphiregulin Protéine recombinante Ag8907
• Nom complet: amphiregulin
• Masse moléculaire calculée: 252 aa, 28 kDa
• Poids moléculaire observé: 50 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC009799
• Symbole du gène: AREG
• Identification du gène (NCBI): 374
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Publications

Species	Application	Title
human	IF	Cell Rep Med; Tumor-associated monocytes promote mesenchymal transformation through EGFR signaling in glioma; Authors - Yiyun Chen
human	WB	J Invest Dermatol; LPCAT1 promotes cutaneous squamous cell carcinoma via EGFR-mediated AKT/p38MAPK signaling pathways.; Authors - Yingjian Huang
		Int J Biol Sci; CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17.; Authors - Jie Liu