Anticorps Polyclonal de lapin anti-ARF3

ARF3 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 10800-1-AP

Synonymes

ADP ribosylation factor 3, ARF3

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Galerie de données de validation

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  WB analysis of mouse brain using 10800-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 10800-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10800-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10800-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10800-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10800-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10800-1-AP (ARF3 antibody) at dilution of 1:10 (under 10x lens).

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  IF Staining of HepG2 using 10800-1-AP

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10800-1-AP

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules HepG2, cellules Raji, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du pancréas humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

10800-1-AP cible ARF3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARF3 Protéine recombinante Ag1174
• Nom complet: ADP-ribosylation factor 3
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC017565
• Symbole du gène: ARF3
• Identification du gène (NCBI): 377
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
mouse	WB,IHC	Development; PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis; Authors - Naila Umer