Anticorps Monoclonal anti-ARF4

• Phare
• Validé par KD/KO

ARF4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2A4B8

N° de cat : 68204-1-Ig

Synonymes

ARF2, ADP-ribosylation factor 4, ADP ribosylation factor 4, 2A4B8

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Galerie de données de validation

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  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 68204-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68204-1-Ig

  WB result of ARF4 antibody (68204-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF4 transfected HeLa cells.

• 

  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68204-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 68204-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68204-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68204-1-Ig (ARF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 68204-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 68204-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68204-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 68204-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68204-1-Ig (ARF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68204-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARF4 antibody (68204-1-Ig, Clone: 2A4B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 68204-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ARF4 (68204-1-Ig, Clone:2A4B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du pancréas humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

68204-1-Ig cible ARF4 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARF4 Protéine recombinante Ag25522
• Nom complet: ADP-ribosylation factor 4
• Masse moléculaire calculée: 180 aa, 21 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC003364
• Symbole du gène: ARF4
• Identification du gène (NCBI): 378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARF4, also named as ARF2, belongs to the small GTPase superfamily and Arf family. ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. Their function are not fully described. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publications

Species	Application	Title
rat	WB	Cell Signal; Targeting CDK1 inhibits Golgi apparatus stress-mediated neuroinflammation and neuronal apoptosis after intracerebral hemorrhage by modulating GRASP55 phosphorylation; Authors - Xiaobo Zhang

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