Anticorps Polyclonal de lapin anti-ARF5-Specific

• Phare
• Validé par KD/KO

ARF5-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20227-1-AP

Synonymes

ARF5, ADP-ribosylation factor 5, ADP ribosylation factor 5

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 20227-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20227-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20227-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20227-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20227-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20227-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20227-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20227-1-AP (ARF5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 20227-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 20227-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20227-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20227-1-AP (ARF5-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, cellules HeLa, tissu cérébral de souris, tissu de côlon de souris, tissu hépatique de souris, tissu rénal de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

20227-1-AP cible ARF5-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ADP-ribosylation factor 5
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: NM_001662
• Symbole du gène: ARF5
• Identification du gène (NCBI): 381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF4 and ARF5 are class II ARFs. (PMID: 7759471, PMID: 16042562). Their function are not fully described.The antibody is specific to ARF5.

Publications

Species	Application	Title
mouse	IF	PeerJ; Effective protein inhibition in intact mouse oocytes through peptide nanoparticle-mediated antibody transfection.; Authors - Ruichao Li
mouse	WB	Cell Rep; Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis; Authors - Rakhee Bajaj ; KD Validated
human	WB	Front Oncol; Acute aerobic exercise alters serum protein distribution in colorectal cancer patients; Authors - Sinan Jin

{{ptg:RelatedPrimaryAntibodies}}