Anticorps Monoclonal anti-ARF6

ARF6 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H5H1

N° de cat : 68924-1-PBS

Synonymes

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

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Galerie de données de validation

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  WB analysis of LNCaP using 68924-1-Ig (same clone as 68924-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68924-1-Ig (same clone as 68924-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 68924-1-Ig (same clone as 68924-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68924-1-Ig (same clone as 68924-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68924-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68924-1-PBS cible ARF6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARF6 Protéine recombinante Ag12642
• Nom complet: ADP-ribosylation factor 6
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC002952
• Symbole du gène: ARF6
• Identification du gène (NCBI): 382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

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