Anticorps Polyclonal de lapin anti-ARFGEF2

ARFGEF2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31277-1-PBS

Synonymes

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2

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Galerie de données de validation

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  WB analysis using 31277-1-AP (same clone as 31277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31277-1-AP (ARFGEF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 31277-1-AP (same clone as 31277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31277-1-AP (ARFGEF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31277-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31277-1-PBS cible ARFGEF2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARFGEF2 Protéine recombinante Ag35396
• Nom complet: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Poids moléculaire observé: 202 kDa
• Numéro d’acquisition GenBank: BC050449
• Symbole du gène: ARFGEF2
• Identification du gène (NCBI): 10564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

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