Anticorps Recombinant de lapin anti-ARFGEF2

ARFGEF2 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241538H5

N° de cat : 84296-4-PBS

Synonymes

BIG2, 241538H5, ARFGEP2, Brefeldin A inhibited GEP 2

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Galerie de données de validation

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  WB analysis using 84296-4-RR (same clone as 84296-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84296-4-RR (same clone as 84296-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84296-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01199-3

  Cytometric bead array standard curve of MP01199-3, ARFGEF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84296-5-PBS. Detection antibody: 84296-4-PBS. Standard: Ag35396. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84296-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

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Informations sur le produit

84296-4-PBS cible ARFGEF2 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARFGEF2 Protéine recombinante Ag35396
• Nom complet: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Poids moléculaire observé: 202 kDa
• Numéro d’acquisition GenBank: BC050449
• Symbole du gène: ARFGEF2
• Identification du gène (NCBI): 10564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

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