Anticorps Polyclonal de lapin anti-ARFRP1

ARFRP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 17712-1-AP

Synonymes

ARP1, ARF-related protein 1, ARF related protein 1, ADP-ribosylation factor-related protein 1, ADP ribosylation factor related protein 1

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Galerie de données de validation

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  WB analysis of Jurkat using 17712-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17712-1-AP (ARFRP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17712-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17712-1-AP (ARFRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 17712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17712-1-AP (ARFRP1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 17712-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17712-1-AP (ARFRP1 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17712-1-AP cible ARFRP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARFRP1 Protéine recombinante Ag12036
• Nom complet: ADP-ribosylation factor related protein 1
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC092480
• Symbole du gène: ARFRP1
• Identification du gène (NCBI): 10139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARFRP1, or ADP-ribosylation factor-related protein 1, is a small GTPase that is significantly similar to the ARF family of proteins. It plays a crucial role in intracellular membrane trafficking, particularly in post-Golgi membrane trafficking processes. ARFRP1 is primarily associated with the trans-Golgi compartment and the trans-Golgi network (TGN), where it functions as an essential regulatory factor for the targeting of Arl1 and GRIP domain-containing proteins, such as golgin-97 and golgin-245, onto Golgi membranes. Furthermore, ARFRP1 works in concert with Arl1 and GRIP proteins to facilitate the transport of the vesicular stomatitis virus G protein from the Golgi to the plasma membrane, as well as the retrograde transport of TGN38 and Shiga toxin from endosomes back to the TGN. This protein is also known to function upstream of ARL1 and ARL5, coordinating the recruitment of distinct tethering factors, golgins, and GARP, to the TGN. This mechanism is essential for the delivery of retrograde cargos to the TGN, making ARFRP1 a master regulator of retrograde-carrier tethering to the TGN.

Publications

Species	Application	Title
human	WB	Anal Chem; Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells; Authors - Zi Gao

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