Anticorps Monoclonal anti-Arginase-1

• Phare
• Validé par KD/KO

Arginase-1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

58

CloneNo.

5D6D12

N° de cat : 66129-1-Ig

Synonymes

ARG1, Arginase 1, arginase, liver, Arginase-1, liver Arginase, Liver type arginase

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Galerie de données de validation

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  WB analysis of rat liver using 66129-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66129-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66129-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66129-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66129-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66129-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66129-1-Ig (ARG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat liver using 66129-1-Ig

  IP result of anti-ARG1 (IP:66129-1-Ig, 5ug; Detection:66129-1-Ig 1:1000) with rat liver tissue lysate 5520 ug.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66129-1-Ig (ARG1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 66129-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (66129-1-Ig, Clone: 5D6D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules RAW 264.7, tissu hépatique de porc, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de rat,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 44 publications below
• IHC: See 4 publications below
• IF: See 23 publications below

Informations sur le produit

66129-1-Ig cible Arginase-1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arginase-1 Protéine recombinante Ag8810
• Nom complet: arginase, liver
• Masse moléculaire calculée: 236aa,25 kDa; 322aa,35 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC005321
• Symbole du gène: ARG1
• Identification du gène (NCBI): 383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

Publications

Species	Application	Title
human,mouse	WB,IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
	WB	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
rat	WB,IF	Int J Biol Macromol; A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects; Authors - Wentao Shi
mouse	WB,IHC,IF	Carbohydr Polym; Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism; Authors - Lu Li
mouse	WB	Int J Nanomedicine; Rosuvastatin Nanomicelles Target Neuroinflammation and Improve Neurological Deficit in a Mouse Model of Intracerebral Hemorrhage.; Authors - Liu Zi
	IF	NPJ Parkinsons Dis; Melatonin ameliorates Parkinson's disease via regulating microglia polarization in a RORα-dependent pathway.; Authors - Jingwen Li