Anticorps Monoclonal anti-Arginase-1

• Phare
• Validé par KD/KO

Arginase-1 Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(5)

CloneNo.

5D6D12

Avis (1)

N° de cat : CL594-66129

Synonymes

ARG1, Arginase 1, liver Arginase, arginase, liver, Arg-1

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-66129

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Arginase-1 antibody (CL594-66129, Clone: 5D6D12 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 5 publications below

Informations sur le produit

CL594-66129 cible Arginase-1 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arginase-1 Protéine recombinante Ag8810
• Nom complet: arginase, liver
• Masse moléculaire calculée: 236aa,25 kDa; 322aa,35 kDa
• Numéro d’acquisition GenBank: BC005321
• Symbole du gène: Arginase-1
• Identification du gène (NCBI): 383
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); Wireless Localized Electrical Stimulation Generated by an Ultrasound-Driven Piezoelectric Discharge Regulates Proinflammatory Macrophage Polarization.; Authors - Ying Kong
	IF	ACS Nano; Biomimetic Trypsin-Responsive Structure-Bridged Mesoporous Organosilica Nanomedicine for Precise Treatment of Acute Pancreatitis; Authors - Yanan Wang
mouse	IF	Adv Sci (Weinh); ROS-Driven Nanoventilator for MRSA-Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti-Inflammation and Immunomodulation; Authors - Zheng Luo
	IF	Adv Sci (Weinh); Natural Enzyme-Loaded Polymeric Stealth Coating-Armed Engineered Probiotics by Disrupting Tumor Lactate Homeostasis to Synergistic Metabolism-Immuno-Enzyme Dynamic Therapy; Authors - Liuzhou Mao
	IF	Adv Sci (Weinh); Mitochondria-Targeted ROS Scavenging Natural Enzyme Cascade Nanogels for Periodontitis Treatment via Hypoxia Alleviation and Immunomodulation; Authors - Yanfen Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachel (Verified Customer) (08-09-2022)

This ab works really well for IF in murine liver samples (formalin-fixed).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse liver

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