Anticorps Recombinant de lapin anti-ARHGAP4

ARHGAP4 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242995B5

N° de cat : 85648-2-PBS

Synonymes

KIAA0131, p115, RGC1, Rho GTPase-activating protein 4, Rho-GAP hematopoietic protein C1

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Galerie de données de validation

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  WB analysis using 85648-2-RR (same clone as 85648-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85648-2-RR (ARHGAP4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85648-2-RR (same clone as 85648-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85648-2-RR (ARHGAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85648-2-RR (same clone as 85648-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85648-2-RR (ARHGAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85648-2-RR (same clone as 85648-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (85648-2-RR, Clone: 242995B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85648-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85648-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85648-2-RR against Human ARHGAP4 were performed. The affinity constant is 0.518 nM.

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Informations sur le produit

85648-2-PBS cible ARHGAP4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARHGAP4 Protéine recombinante Ag10091
• Nom complet: Rho GTPase activating protein 4
• Masse moléculaire calculée: 986 aa, 109 kDa
• Poids moléculaire observé: 115-120 kDa
• Numéro d’acquisition GenBank: BC052303
• Symbole du gène: ARHGAP4
• Identification du gène (NCBI): 393
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARHGAP4 (Rho GTPase-Activating Protein 4), also named as KIAA0131, RGC1, RHOGAP4, is a member of the RhoGAP family, which regulates Rho GTPases by accelerating their hydrolysis of GTP to GDP, thereby inactivating these signaling molecules. Rho GTPases are critical regulators of cytoskeletal dynamics, cell motility, and intracellular trafficking.

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