Anticorps Polyclonal de lapin anti-ARHGAP5

ARHGAP5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 55165-1-AP

Synonymes

ARHGAP5, GFI2, p190 B, RhoGAP5

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Galerie de données de validation

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  WB analysis using 55165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

55165-1-AP cible ARHGAP5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Rho GTPase activating protein 5
• Masse moléculaire calculée: 172 kDa
• Poids moléculaire observé: 170-190 kDa
• Numéro d’acquisition GenBank: NM_001173
• Symbole du gène: ARHGAP5
• Identification du gène (NCBI): 394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARHGAP5, also named as RHOGAP5 and p190-B, is GTPase-activating protein for Rho family members. It may play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. ARHGAP5 can be directly phosphorylated on a single identified tyrosine residue by the activated INS and IGF-1 receptors. It also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of ARHGAP5 is shown to attenuate signal transducing activity of Rac, Rho and CDC42. The antibody is specific to ARHGAP5.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis.; Authors - Ruli Li
human	WB	Nat Metab; Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis.; Authors - Camille Arcinas

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

Good, both for WB and IF.By WB, although there are some non-specific bands, it can detect endogenous protein; by IF, PFA fixation, it labels the protein in the Golgi and the nucleus.

• Applications: Western Blot, Immunofluorescence,