Anticorps Monoclonal anti-ARHGDIB

ARHGDIB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E7B7

N° de cat : 66054-1-PBS

Synonymes

3E7B7, GDIA2, GDID4, Ly GDI, Ly-GDI

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Galerie de données de validation

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  WB analysis using 66054-1-Ig (same clone as 66054-1-PBS)

  Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66054-1-Ig (same clone as 66054-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66054-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66054-1-PBS cible ARHGDIB dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARHGDIB Protéine recombinante Ag9091
• Nom complet: Rho GDP dissociation inhibitor (GDI) beta
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC009200
• Symbole du gène: ARHGDIB
• Identification du gène (NCBI): 397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

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