Anticorps Monoclonal anti-ARHGEF16

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D8E3

N° de cat : 67975-1-PBS

Synonymes

NBR, GEF16, Ephexin-4, EPHEXIN4, Ephexin 4

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Galerie de données de validation

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  WB analysis using 67975-1-Ig (same clone as 67975-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67975-1-Ig (same clone as 67975-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67975-1-Ig (same clone as 67975-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67975-1-Ig (same clone as 67975-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67975-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67975-1-PBS cible ARHGEF16 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARHGEF16 Protéine recombinante Ag28675
• Nom complet: Rho guanine exchange factor (GEF) 16
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC002681
• Symbole du gène: ARHGEF16
• Identification du gène (NCBI): 27237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

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