Anticorps Recombinant de lapin anti-ARHGEF19

ARHGEF19 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230290F5

N° de cat : 82966-2-PBS

Synonymes

230290F5, Ephexin-2, WGEF

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Galerie de données de validation

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  IF Staining of U2OS using 82966-2-RR (same clone as 82966-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ARHGEF19 antibody (82966-2-RR, Clone: 230290F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 82966-2-RR (same clone as 82966-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82966-2-RR (same clone as 82966-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human ARHGEF19 (82966-2-RR, Clone:230290F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Control Antibody(blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82966-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82966-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82966-2-RR against Human ARHGEF19 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82966-2-PBS cible ARHGEF19 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARHGEF19 Protéine recombinante Ag11645
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 19
• Masse moléculaire calculée: 802 aa, 89 kDa
• Numéro d’acquisition GenBank: BC040640
• Symbole du gène: ARHGEF19
• Identification du gène (NCBI): 128272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rho guanine nucleotide exchange factors (RHOGEFs) are proteins that could regulate Rho GTPases, which can be involved in a variety of physiological processes, such as survival, development, differentiation, and motility. ARHGEF19 also known as WGEF, is a member of the RhoGEF family. ARHGEF19 contains two identical functional domains, including the Dbl homology (DH) and pleckstrin homology (PH) domain(PMID:32993957). ARHGEF19 was highly expressed in human breast cancer(PMID: 34813497).

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