Anticorps Polyclonal de lapin anti-ARHGEF5

ARHGEF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11379-1-PBS

Synonymes

Ephexin-3, GEF5, Oncogene TIM, P60, p60 TIM

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Galerie de données de validation

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  WB analysis of MCF-7 using 11379-1-AP (same clone as 11379-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 11379-1-AP (same clone as 11379-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11379-1-AP (same clone as 11379-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 11379-1-AP (same clone as 11379-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11379-1-AP (same clone as 11379-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11379-1-AP (same clone as 11379-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11379-1-AP (same clone as 11379-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)

  IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11379-1-AP (same clone as 11379-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARHGEF5 antibody (11379-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11379-1-PBS cible ARHGEF5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARHGEF5 Protéine recombinante Ag1892
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 5
• Masse moléculaire calculée: 177 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC010046
• Symbole du gène: ARHGEF5
• Identification du gène (NCBI): 7984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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