Anticorps Polyclonal de lapin anti-ARID1A

ARID1A Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 30304-1-AP

Synonymes

BM029, BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A

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Galerie de données de validation

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  WB analysis using 30304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30304-1-AP

  IP result of anti-ARID1A (IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

30304-1-AP cible ARID1A dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARID1A Protéine recombinante Ag30749
• Nom complet: AT rich interactive domain 1A (SWI-like)
• Masse moléculaire calculée: 242 kDa
• Poids moléculaire observé: 250-260 kDa
• Numéro d’acquisition GenBank: NM_006015
• Symbole du gène: ARID1A
• Identification du gène (NCBI): 8289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

Publications

Species	Application	Title
human	WB	Acta Biomater; Effective Delivery of CRISPR/dCas9-SAM for Multiplex Gene Activation Based on Mesoporous Silica Nanoparticles for Bladder Cancer Therapy; Authors - Jinming Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Great antibody that detects ARID1A by WB from HeLa extracts (1/1000 dilution, 1h at room temperature)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

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