Anticorps Polyclonal de lapin anti-ARID1A

ARID1A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30304-1-PBS

Synonymes

BM029, BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A

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Galerie de données de validation

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  WB analysis using 30304-1-AP (same clone as 30304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 30304-1-AP (same clone as 30304-1-PBS)

  IP result of anti-ARID1A (IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 30304-1-AP (same clone as 30304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30304-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30304-1-PBS cible ARID1A dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARID1A Protéine recombinante Ag30749
• Nom complet: AT rich interactive domain 1A (SWI-like)
• Masse moléculaire calculée: 242 kDa
• Poids moléculaire observé: 250-260 kDa
• Numéro d’acquisition GenBank: NM_006015
• Symbole du gène: ARID1A
• Identification du gène (NCBI): 8289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

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