Anticorps Recombinant de lapin anti-ARID1A

ARID1A Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240690F1

N° de cat : 83733-3-RR

Synonymes

BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A

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Galerie de données de validation

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  WB analysis of BxPC-3 using 83733-3-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83733-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 83733-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 83733-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 83733-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83733-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83733-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules BxPC-3, cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83733-3-RR cible ARID1A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARID1A Protéine recombinante Ag30749
• Nom complet: AT rich interactive domain 1A (SWI-like)
• Masse moléculaire calculée: 242 kDa
• Poids moléculaire observé: 250-260 kDa
• Numéro d’acquisition GenBank: NM_006015
• Symbole du gène: ARID1A
• Identification du gène (NCBI): 8289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

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