Anticorps Recombinant de lapin anti-ARID1B

ARID1B Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230222G4

N° de cat : 82979-1-PBS

Synonymes

230222G4, BAF250b, BRIGHT, DAN15, ELD/OSA1

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Galerie de données de validation

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  IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARID1B antibody (82979-1-RR, Clone: 230222G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82979-1-RR (same clone as 82979-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARID1B antibody (82979-1-RR, Clone: 230222G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 82979-1-RR (same clone as 82979-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-HUMAN ARID1B (82979-1-RR, Clone:230222G4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82979-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82979-1-RR against Human ARID1B were performed. The affinity constant is 0.606 nM.

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Informations sur le produit

82979-1-PBS cible ARID1B dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARID1B Protéine recombinante Ag30643
• Nom complet: AT rich interactive domain 1B (SWI1-like)
• Masse moléculaire calculée: 236 kDa
• Numéro d’acquisition GenBank: NM_017519
• Symbole du gène: ARID1B
• Identification du gène (NCBI): 57492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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