Anticorps Polyclonal de lapin anti-ARID3A

• Phare
• Validé par KD/KO

ARID3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14068-1-AP

Synonymes

ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1

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Galerie de données de validation

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  WB analysis using 14068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14068-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14068-1-AP

  IP Result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IP experiment of K-562 using 14068-1-AP

  IP result of anti-ARID3A(IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14068-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARID3A antibody (14068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of HepG2 using 14068-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu placentaire de souris
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

14068-1-AP cible ARID3A dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARID3A Protéine recombinante Ag5124
• Nom complet: AT rich interactive domain 3A (BRIGHT-like)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC060828
• Symbole du gène: ARID3A
• Identification du gène (NCBI): 1820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	IHC	PLoS Genet; Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.; Authors - Blair B Madison
human	WB,IP	Carcinogenesis; ARID3A promotes the development of colorectal cancer by upregulating AURKA.; Authors - Jing Tang ; KD Validated
human	WB,IHC	Ann Surg Oncol; High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.; Authors - Meiying Song
human,mouse	WB,IF	Cell Biol Int; ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells; Authors - Xudong Guo ; KD Validated
human	WB,ChIP	Cell Biol Int; ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3.; Authors - Yafei Li