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Anticorps Monoclonal anti-ARID3A

ARID3A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B10C10

N° de cat : 68428-1-PBS

Synonymes

Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A, ARID domain-containing protein 3A

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARID3A Monoclonal antibody (68428-1-Ig)

    WB analysis using 68428-1-Ig (same clone as 68428-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68428-1-Ig (ARID3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ARID3A Monoclonal antibody (68428-1-Ig)

    IF Staining of K-562 using 68428-1-Ig (same clone as 68428-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ARID3A antibody (68428-1-Ig, Clone: 2B10C10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68428-1-PBS in a different storage buffer formulation.

Informations sur le produit

68428-1-PBS cible ARID3A dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène ARID3A Protéine recombinante Ag7655
Nom complet AT rich interactive domain 3A (BRIGHT-like)
Masse moléculaire calculée 63 kDa
Poids moléculaire observé 75 kDa
Numéro d’acquisition GenBankBC060828
Symbole du gène ARID3A
Identification du gène (NCBI) 1820
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

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