Anticorps Polyclonal de lapin anti-ARID4B

• Phare
• Validé par KD/KO

ARID4B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 24499-1-AP

Synonymes

RBBP1L1, Breast cancer-associated antigen BRCAA1, BRCAA1, AT-rich interactive domain-containing protein 4B, ARID domain-containing protein 4B

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Galerie de données de validation

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  WB analysis of MCF-7 using 24499-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24499-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24499-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 24499-1-AP

  IP result of anti-ARID4B (IP:24499-1-AP, 5ug; Detection:24499-1-AP 1:1000) with MCF-7 cells lysate 4000ug.

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  IHC staining of human cervical cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 24499-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARID4B antibody (24499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules K-562
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

24499-1-AP cible ARID4B dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARID4B Protéine recombinante Ag21462
• Nom complet: AT rich interactive domain 4B (RBP1-like)
• Masse moléculaire calculée: 1312 aa, 148 kDa
• Poids moléculaire observé: 100 kDa, 200 kDa
• Numéro d’acquisition GenBank: BC130418
• Symbole du gène: ARID4B
• Identification du gène (NCBI): 51742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Publications

Species	Application	Title
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
human	WB	Theranostics; SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.; Authors - Ti-Chun Chan
human	WB	Cell Death Dis; DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways.; Authors - Xiangxiang Liu
mouse	WB	Br J Nutr; Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway.; Authors - Qi Hao ; KD Validated
human	WB,IP	Cancer Lett; Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance; Authors - Zhaohan Zhang

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