Anticorps Monoclonal anti-ARID4B

ARID4B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB

Conjugaison

Non conjugué

CloneNo.

1H3E2

N° de cat : 67384-1-Ig

Synonymes

ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180

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Galerie de données de validation

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  WB analysis of T-47D using 67384-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 67384-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67384-1-Ig cible ARID4B dans les applications de WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ARID4B Protéine recombinante Ag21485
• Nom complet: AT rich interactive domain 4B (RBP1-like)
• Masse moléculaire calculée: 1312 aa, 148 kDa
• Numéro d’acquisition GenBank: BC130418
• Symbole du gène: ARID4B
• Identification du gène (NCBI): 51742
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.