Anticorps Recombinant de lapin anti-ARL13B

ARL13B Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H5

N° de cat : 82834-2-PBS

Synonymes

3H5, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1

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Galerie de données de validation

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  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 82834-2-RR (same clone as 82834-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using ARL13B antibody (82834-2-RR, Clone: 3H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 82834-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82834-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82834-2-RR against Human ARL13B were performed. The affinity constant is 1.25 nM.

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Informations sur le produit

82834-2-PBS cible ARL13B dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ARL13B Protéine recombinante Ag12015
• Nom complet: ADP-ribosylation factor-like 13B
• Masse moléculaire calculée: 48 kDa
• Numéro d’acquisition GenBank: BC094725
• Symbole du gène: ARL13B
• Identification du gène (NCBI): 200894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.

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