Anticorps Polyclonal de lapin anti-ARL8B

• Phare
• Validé par KD/KO

ARL8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(28)

Avis (1)

N° de cat : 13049-1-AP

Synonymes

ADP-ribosylation factor-like protein 10C, ADP-ribosylation factor-like protein 8B, ARL10C, EC:3.6.5.2, GIE1

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Galerie de données de validation

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  WB analysis of fetal human brain using 13049-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13049-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13049-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL8B antibody (13049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules NIH/3T3, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu d'estomac de rat, tissu pancréatique de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• CoIP: See 1 publications below

Informations sur le produit

13049-1-AP cible ARL8B dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARL8B Protéine recombinante Ag3711
• Nom complet: ADP-ribosylation factor-like 8B
• Masse moléculaire calculée: 186 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC013131
• Symbole du gène: ARL8B
• Identification du gène (NCBI): 55207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Lysosomal positioning coordinates cellular nutrient responses.; Authors - Korolchuk Viktor I VI ; KD Validated
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
rat	IF	Nat Commun; Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons; Authors - Chun Hei Li ; KD Validated
monkey	WB	Sci Adv; The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.; Authors - Meng Lu ; KD Validated
mouse	WB,IF	Sci Adv; Endosomal sorting drives the formation of axonal prion protein endoggresomes.; Authors - Romain Chassefeyre ; KO Validated
human	WB	Nat Commun; RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.; Authors - Tal Keren-Kaplan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olivia (Verified Customer) (01-08-2020)

WB: Lysates from mouse bone marrow-derived macrophages were subjected to SDS PAGE followed by western blot with 13049-1-AP(Arl8b antibody) at a dilution of 1:500 overnight at 4°C.IF: Mouse bone marrow-derived macrophages were fixed in 4% PFA and permeabilized with 0.5% saponin before incubation with 1:500 Arl8b antibody (in 1% BSA/0.1% saponin/PBS) overnight at 4°C, followed by incubation with anti-rabbit Alexa Fluor 568 for 1h at room temperature.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse bone marrow-derived macrophages

ARL8B Antibody Western Blot,Immunofluorescence, validation (1:500 dilution) in Mouse bone marrow-derived macrophages (Cat no:13049-1-AP)

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