Anticorps Polyclonal de lapin anti-ARMC8

• Phare
• Validé par KD/KO

ARMC8 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (2)

N° de cat : 12653-1-AP

Synonymes

armadillo repeat containing 8, ARMC8, HSPC056, S863 2

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Galerie de données de validation

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  WB analysis using 12653-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

12653-1-AP cible ARMC8 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARMC8 Protéine recombinante Ag3374
• Nom complet: armadillo repeat containing 8
• Masse moléculaire calculée: 673 aa, 75.5 kDa
• Poids moléculaire observé: 75.5 kDa
• Numéro d’acquisition GenBank: BC013424
• Symbole du gène: ARMC8
• Identification du gène (NCBI): 25852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Armadillo repeat-containing protein 8 (Armc8) is a key factor to regulate cell membrane adhesion complex through promoting α-catenin degradation. Armc8 expression was elevated during atypia-to-carcinoma progression and may be associated with cancer development of breast carcinoma (PMID: 25119601).

Publications

Species	Application	Title
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB, IHC	J Dermatol Sci; Downregulation of ARMC8 promotes tumorigenesis through activating Wnt/β-catenin pathway and EMT in cutaneous squamous cell carcinomas.; Authors - Xiangzhi Li
human	WB	Dose Response; MiR-664 Protects Against UVB Radiation-Induced HaCaT Cell Damage via Downregulating ARMC8.; Authors - Chen Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (12-31-2021)

This antibody works quite well. There are a few off target bands though, if the samples are loaded too much.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

ARMC8 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:12653-1-AP)

FH

H (Verified Customer) (09-27-2020)

This antibody detects both ARMC8a and b. The signal was good enough to tell which are the right bands immediately.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

ARMC8 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:12653-1-AP)