Anticorps Polyclonal de lapin anti-ARMCX3

• Phare
• Validé par KD/KO

ARMCX3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25705-1-AP

Synonymes

UNQ2517/PRO6007, Protein ALEX3, KIAA0443, BM-017, Armadillo repeat-containing X-linked protein 3

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Galerie de données de validation

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  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of fetal human brain using 25705-1-AP

  IP result of anti-ARMCX3 (IP:25705-1-AP, 1:500; Detection:25705-1-AP, 1:3000) with human brain lysate, 150 ug. Courtesied by Dr. Mirra and Dr. Manso of University of Barcelona.

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  IHC staining of human ovary cancer using 25705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25705-1-AP (ARMCX3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: cerveau humain fœtal
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below

Informations sur le produit

25705-1-AP cible ARMCX3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARMCX3 Protéine recombinante Ag22501
• Nom complet: armadillo repeat containing, X-linked 3
• Masse moléculaire calculée: 379 aa, 43 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC005194
• Symbole du gène: ARMCX3
• Identification du gène (NCBI): 51566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARMCX3, also named as Armadillo repeat-containing X-linked protein 3, is a 379 amino acid protein, which contains 3 ARM repeats. ARMCX3 is a single pass membrane protein belonging to the armadillo repeat family of proteins. ARMCX3 is believed to play a role in embryonic development and tissue maintenance and may also function as a tumor suppressor.

Publications

Species	Application	Title
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
	WB	Sci Signal; A mammalian-specific Alex3/Gαq protein complex regulates mitochondrial trafficking, dendritic complexity, and neuronal survival; Authors - Ismael Izquierdo-Villalba ; KO Validated
human	WB	Cancers (Basel); ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity.; Authors - Serena Mirra
mouse	WB	Int J Obes (Lond); The armadillo-repeat containing X-linked protein 3, ARMCX3, is a negative regulator of the browning of adipose tissue associated with obesity.; Authors - Aleix Gavaldà-Navarro
human	WB	Heliyon; ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells; Authors - Quanying Zhou

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